Basics of High-Performance Liquid Chromatography (HPLC)

Course unit code

Specialization 04.03.01 – Chemistry

Course unit title

Basics of High-Performance Liquid Chromatography (HPLC)

Name(s), surname(s) and title of lecturer(s)

Victor Khasanov

Level of course

Master

Semester

2

ECTS credits

3

Working hours

Contact hours

36

lectures

18

seminars

18

practical classes

laboratory classes

consultations

Independent work

72

Total

108

Work placement

none

Language of instruction

English

Prerequisites

Inorganic Chemistry, Organic Chemistry, Physical Chemistry, Analytical Ch.

Objectives of the course 

 Learning outcomes

 A student’s assessments methods

aims:

-to study the basic aspects of the human resource management;

- to study principles, methods and mechanisms of human resource management;

- to study the modern staff technologies;

- to study the specificityof formation of staff policy 

Formation of competence:

а) intercultural:

- the ability to develop cultural and professional level independently and to master new methods of research;

- the ability to get and use new knowledge independently.

b) professional:

- the ability to develop and construct protocols for HPLC separations;

- receiving of professional skills in HPLC operation;

- ability to organize and manage an HPLC laboratory

Presentations, tests and written exam.

Teaching methods

Lectures, group work, delivery of individual presentations, independent study of literature, case studies, role plays, brainstorming.

Course unit content

Title

Lecturers  (hours)

Self-study (hours)

Theoretical aspects of liquid chromatography and history

2

2

modern variations of HPLC

4

6

Apparatus for HPLC technique, main components

6

6

Types of detecting system

4

4

Types of column packings

4

6

Modes of HPLC separation 1: RP-HPLC

4

6

Modes of HPLC separation 2: normal-phase and other HPLC versions (ion-exchange, gel-permeation, etc.)

4

16

Samples and separation media preparation

4

12

HPLC lab accessories and equipment

4

16

36

72

Assessment requirements

Student’s skills in this subject will be evaluated by means of presentations in the seminars, written tests and final examination.

Assessment criteria

The assessment of the presented works is carried out by the following criteria:

– compliance of the contents to a subject;

– existence of logical communication of stated information;

– compliance of registration to requirements;

– accuracy and literacy of a statement;

– work is handed over in time.

The composition of final accumulative mark

Final accumulative mark consists of:  2 tests –15 % each, individual assignment – 20 %, exam –  50 %

Course outline arranged by