Biochemistry and Bioprocess (FPLC and it‘s use in Biotechnology)

Facts  
Duration: 1 semester
Period: Spring (2) Semester
Credits: 3 ECTS
Contact Hours: 36
Self-study: 72
Hours: 108

Main Objectives

  • to study the basic aspects of the human resource management;
  • to study principles, methods and mechanisms of human resource management;
  • to study the modern staff technologies;
  • to study the specificityof formation of staff policy. 

Learning Outcomes

Formation of competence:

а) intercultural:

  • the ability to develop cultural and professional level independently and to master new methods of research;
  • the ability to get and use new knowledge independently.

b) professional:

  • understanding of an LC for biomolecules separation and purification; an FPLC
  • the ability to develop and construct protocols for FPLC separations;
  • receiving of professional skills in FPLC operation; media handling, column packing, separations performing;
  • ability to organize and manage a Bioprocess laboratory

Professor

Victor Khasanov

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Course annotation

Course unit code

Specialization 04.03.01 – Chemistry

Course unit title

Biochemistry and Bioprocess (FPLC and it‘s use in Biotechnology)

Name(s), surname(s) and title of lecturer(s)

Victor Khasanov

Level of course

Master

Semester

2

ECTS credits

3

Working hours

Contact hours

36

lectures

18

seminars

18

practical classes

laboratory classes

consultations

Independent work

72

Total

108

Work placement

none

Language of instruction

English

Prerequisites

Inorganic Chemistry, Organic Chemistry, Physical Chemistry, Analytical Ch.

Objectives of the course 

 Learning outcomes

 A student’s assessments methods

aims:

-to study the basic aspects of the human resource management;

- to study principles, methods and mechanisms of human resource management;

- to study the modern staff technologies;

- to study the specificityof formation of staff policy 

Formation of competence:

а) intercultural:

the ability to develop cultural and professional level independently and to master new methods of research;

- the ability to get and use new knowledge independently.

b) professional:

- understanding of an LC for biomolecules separation and purification; an FPLC

the ability to develop and construct protocols for FPLC separations;

- receiving of professional skills in FPLC operation; media handling, column packing, separations performing;

- ability to organize and manage a Bioprocess laboratory

Presentations, tests and written exam.

Teaching methods

Lectures, group work, delivery of individual presentations, independent study of literature, case studies, role plays, brainstorming.

Course unit content

Title

Lecturers  (hours)

Self-study (hours)

Theoretical aspects of liquid chromatography in biochemistry (HPLC DfB and FPLC)

2

4

Modern variations of FPLC

4

8

Apparatus for HPLC technique, main components

6

8

Types and scales of columns and packings

6

14

Water preparation and eluent handling

4

8

Sample treatment and fraction collection

4

8

Modes of FPLC separation: ion-exchange, gel-filtration, HIC, affinity, etc.)

6

14

FPLC and Bioprocess lab accessories and equipment

4

8

36

72

Assessment requirements

Student’s skills in this subject will be evaluated by means of presentations in the seminars, written tests and final examination.

Assessment criteria

The assessment of the presented works is carried out by the following criteria:

– compliance of the contents to a subject;

– existence of logical communication of stated information;

– compliance of registration to requirements;

– accuracy and literacy of a statement;

– work is handed over in time.

The composition of final accumulative mark

Final accumulative mark consists of:  2 tests –15 % each, individual assignment – 20 %, exam –  50 %

Course outline arranged by